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Work out the concentration of a reconstituted lyophilized peptide and the volume of solution that contains a given amount. Concentration in mg/mL is numerically the same as µg/µL, which makes small aliquots easy to plan.
Concentration
5 mg/mL
= 5 µg/µL · 5,000 µg/mL
Volume for the target amount
50 µL
= 0.05 mL of solution
Aliquots per vial
40
How the calculation works
Concentration is the mass of peptide divided by the volume of diluent: C (mg/mL) = mass (mg) ÷ volume (mL). Because 1 mg/mL equals 1 µg/µL, the volume that contains a target amount is simply the target in micrograms divided by the concentration: V (µL) = target (µg) ÷ C (mg/mL). The number of whole aliquots is the total mass in micrograms divided by the target, rounded down.
Worked example
A vial containing 10 mg of lyophilized peptide is reconstituted with 2 mL of bacteriostatic water. The concentration is 10 ÷ 2 = 5 mg/mL, which is 5 µg/µL. An aliquot of 250 µg therefore needs 250 ÷ 5 = 50 µL of solution, and the vial yields 10,000 ÷ 250 = 40 aliquots.
Accuracy notes for the lab
▪
The label states the gross fill. Net peptide content, the actual peptide after counter-ions and residual water, is usually lower. Use the net peptide value from the Certificate of Analysis when exact molar amounts matter.
▪
Pipette the diluent with a calibrated pipette rather than estimating from vial graduations, and let the cake dissolve fully before drawing an aliquot.
▪
Choose a volume that gives an easy-to-measure concentration. Very small aliquot volumes amplify pipetting error.
For laboratory research use only. This calculator performs unit conversions for in-vitro work. It is not a dosing tool and must not be used to prepare anything for administration to humans or animals.
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Work out the concentration of a reconstituted lyophilized peptide and the volume of solution that contains a given amount. Concentration in mg/mL is numerically the same as µg/µL, which makes small aliquots easy to plan.
Concentration
{{ calcConcStr }}
{{ calcConcAlt }}
Volume for the target amount
{{ calcVolStr }}
{{ calcVolAlt }}
Aliquots per vial
{{ calcAliquotStr }}
{{ calcErr }}
How the calculation works
Concentration is the mass of peptide divided by the volume of diluent: C (mg/mL) = mass (mg) ÷ volume (mL). Because 1 mg/mL equals 1 µg/µL, the volume that contains a target amount is simply the target in micrograms divided by the concentration: V (µL) = target (µg) ÷ C (mg/mL). The number of whole aliquots is the total mass in micrograms divided by the target, rounded down.
Worked example
A vial containing 10 mg of lyophilized peptide is reconstituted with 2 mL of bacteriostatic water. The concentration is 10 ÷ 2 = 5 mg/mL, which is 5 µg/µL. An aliquot of 250 µg therefore needs 250 ÷ 5 = 50 µL of solution, and the vial yields 10,000 ÷ 250 = 40 aliquots.
Accuracy notes for the lab
▪
The label states the gross fill. Net peptide content, the actual peptide after counter-ions and residual water, is usually lower. Use the net peptide value from the Certificate of Analysis when exact molar amounts matter.
▪
Pipette the diluent with a calibrated pipette rather than estimating from vial graduations, and let the cake dissolve fully before drawing an aliquot.
▪
Choose a volume that gives an easy-to-measure concentration. Very small aliquot volumes amplify pipetting error.
For laboratory research use only. This calculator performs unit conversions for in-vitro work. It is not a dosing tool and must not be used to prepare anything for administration to humans or animals.
Certificates of analysis
The lab report behind every vial.
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Holding a vial? The lot number is printed on its label.
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